Cytokine quantification in sera of immunised mice. For each cytokine quantified, multiplex ELISA was performed using sera from mice in the test group (Nc-aD-Sf9), the positive control groups (Nc-aD-E. coli and Engerix B) and the negative control groups (Buffer only, Buffer and alum, Nc-Sf9 and Nc-E. coli). The symbols * and ** indicate p < 0.001 and p < 0.0001, respectively, when cytokine concentrations from the test group (Nc-aD-Sf9) and positive control groups (Nc-aD-E. coli and Engerix B) are compared to that of the ‘Buffer only’ negative control group. The error bars represent the mean (±) standard deviation. Nc-aD-Sf9: Nc-aD produced in Sf9 cells, Nc-aD-E. coli: Nc-aD produced in E. coli, Nc-Sf9: Nc produced in Sf9 cells, and Nc-E. coli: Nc produced in E. coli.