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. 2021 Feb 15;12(2):277. doi: 10.3390/genes12020277

Figure 4.

Figure 4

Relative activity of co-expressed wild-type and mutant TAP53 or ΔNP63 proteins compared to the corresponding single protein expression. (A) Comparison of wild-type and mutant TAP53 co-expression activity (i.e., constitutive pADH1-wild-type TAP53 + inducible pGAL1,10 TAP53 R175H or R282W) with that of wild-type TAP53 expressed alone (i.e., constitutive pADH1-wild-type TAP53) in presence of the P53 RE from the PUMA target gene (yLFM-PUMA) or the same p53 RE along with a G4 prone sequence upstream (yLFM-KSHV-PUMA) or downstream (yLFM-PUMA-KSHV). (B) Comparison of wild-type and mutant ΔNP63 co-expression activity (i.e., constitutive pADH1-wild-type ΔNP63 + inducible pGAL1,10 ΔNP63 G134V or R204W) with that of wild-type ΔNP63 expressed alone (i.e., constitutive pADH1 wild-type ΔNP63) in presence of the P53 RE from the PUMA target gene (yLFM-PUMA) or the same P53 RE along with a G4 prone sequence upstream (yLFM-KSHV-PUMA) or downstream (yLFM-PUMA-KSHV). WT, wild-type. ns: not significant, ** p < 0.01; *** p < 0.001; **** p < 0.0001.