Table 2.

Details of the two sets of primers and probes used for amplification of the NDV gene fragment in real-time reverse transcriptase polymerase chain reaction (RRT-PCR) and RT-PCR.

Primer	Sequence (5’–3’) Direction	Product Size (bp)	Reference
F+4839	TCCGGAGGATACAAGGGTCT	101	[41]
F+4894 (VFP-1)	[FAM]AAGCGTTTCTGTCTCCTTCCTCCA[TAMRA]
F-4939	AGCTGTTGCAACCCCAAG
NDV-F330 forward	AGGAAGGAGACAAAAACGTTTTATAGG	370	[42]
NDV-R700 reverse	TCAGCTGAGTTAATGCAGGGGAGG