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. 2021 Mar 1;21:207. doi: 10.1186/s12885-021-07857-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Validation of microarray data by RT-qPCR. a Microarray data of the 10 paired adenoma/adenocarcinoma paired samples, showing the upregulation of seven genes in adenocarcinomas compared to adenomas. b RT-qPCR results showing the validation of the microarray results of the same set of genes in a larger set of samples (25 adenoma and 16 adenocarcinoma samples, of which 10 adenoma/adenocarcinoma paired samples and 15 adenoma and 6 adenocarcinoma additional new samples). c and d Relative expression of the RETNLB gene found downregulated in adenocarcinomas when compared to adenomas by microarray (10 paired adenoma-adenocarcinoma samples) and RT-qPCR (10 adenoma/adenocarcinoma paired samples plus 15 adenoma and 6 adenocarcinoma samples) analysis, respectively. Fold changes (log2 FC) between the expression means in adenomas and adenocarcinomas were A = 6.75, B=4.93, C=8.50 and D=11.12. Mann-Whitney’s test was used for statistical analysis (A and B: ***p < 0.0006; C and D: ***p < 0.0001)