Fig. 5.
Knockdown of the ETV4 gene reduced the clonogenic ability and cell migration in the CRC cell lines HT29 and SW480. a Representative images of cell colonies formed after 12 days of culture. b Bar graph representing the mean of three independent experiments. CRC cells were sparsely plated in 6 well plates (500 cells/well), allowed to grow for 12 days, fixed and stained for manual counting (HT29: p=0.0402; SW480: p=0.0015, Mann-Whitney statistical test). c Representative images of cell migration. d Bar graph representing the mean of three independent experiments. Cells were seeded on the top of the insert (1 × 105), fixed 24 h, and stained for manual counting (HT29: p= 0.0231; SW480: p=0.0152, Mann-Whitney statistical test)