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Glycerol–phosphate shuttle. GAPDH generates NADH during glycolysis. NADH can be regenerated to NAD+ to allow glycolysis to continue by the conversion of dihydroxyacetone phosphate into glycerol 3-phosphate by cytosolic glycerol 3-phosphate dehydrogenase. Subsequently, glycerol 3-phosphate is converted back into dihydroxyacetone phosphate by coupling FAD into FADH2. FADH2 donates electrons to ubiquinone, which feeds electrons into complex III of the ETC.
