Table 1. Macromolecule-production information.

	UnGlnK	AdGlnK
Source organism	C. glutamicum	C. glutamicum
DNA source	C. glutamicum genomic DNA	C. glutamicum genomic DNA
Forward primer	GCCAATTGTACCATATGAGCTTGCATGCCTGC†	CCATGCGGATTAAAGGGCTGCTTCGCCGC‡
Reverse primer	GGGCCGCTCGAGTCATCAAAGGGCTGCTTCGCC§	GCGGCGAAGCAGCCCTTTAATCCGCATGG‡
Cloning vector	pZ8-1glnK-Xa-C-Strep	pZ8-1glnK-Xa-C-Strep
Expression vector	pET-15bglnK	pZ8-1glnK
Expression host	E. coli BL21(DE3)	C. glutamicum ATCC 13032
Complete amino-acid sequence of the construct produced	MGSSHHHHHHSSGLVPRGSHMSLHACRMKLITAIVKPFTLTDIKDALEQAGVQGMTVTETQGFGQQKGHTEVYRGAEYAVDFVPKVKIEVIISDAQAEEVINIIVETARTGKVGDGKVWMTNIEELVRVRTGERGEAAL	MKLITAIVKPFTLTDIKDALEQAGVQGMTVTETQGFGQQKGHTEVYRGAEYAVDFVPKVKIEVIISDAQAEEVINIIVETARTGKVGDGKVWMTNIEELVRVRTGERGEAAL

^†The restriction site for NdeI is underlined.

^‡The inserted stop codon is underlined.

^§The restriction site for XhoI is underlined.