| Method |
Sitting-drop vapor diffusion |
Hanging-drop vapor diffusion |
| Plate type |
Greiner CrystalQuick 96-well |
ComboPlate 24-well |
| Temperature (K) |
292 |
292 |
| Protein concentration (mg ml−1) |
13 |
11 |
| Buffer composition of protein solution |
20 mM Tris–HCl pH 8.0, 200 mM NaCl, 1 mM EDTA |
20 mM Tris–HCl pH 7.5, 50 mM NaCl, 2 mM MgCl2
|
| Composition of reservoir solution |
2.0 M ammonium sulfate, 5%(v/v) 2-propanol |
0.06 M MgCl2, 0.06 M CaCl2, 0.1 M MOPS pH 7.1, 0.1 M HEPES pH 7.1, 15%(v/v) MPD, 15%(w/v) PEG 1000, 15%(w/v) PEG 3350 |
| Volume and ratio of drop |
0.4 µl, 1:1 |
2.0 µl, 1:1 |
| Volume of reservoir (µl) |
70 |
700 |
