FIGURE 2.
Quantitative differences of CD4+Foxp3+ regulatory T cells in resting recipient lungs and lung allografts. (A) H&E stained histology pictures, total CD4+ and CD8+ T cell counts in resting lungs of the three different recipient mice. (B) Percentage of Foxp3+ cells among CD4+ T cells in resting lungs. (C) Percentage of Foxp3+ cells among CD4+ T cells in lung grafts harvested at day 7 after engraftment. (D) Expression of CD25 and GITR on CD4+Foxp3+ T cells. (E) Mixed lymphocyte reactions demonstrating inhibition of B6 CD8+ T cell proliferation, as determined by Ki-67 expression, in the presence of Balb/c dendritic cells and Tregs isolated from B6J and B6EMaryland mice. *p < 0.05, **p < 0.01, ***p < 0.001 NS: not significant.
