. Author manuscript; available in PMC: 2021 Mar 1.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Aug 14;1126-1127:121746. doi: 10.1016/j.jchromb.2019.121746

Table 1.

Gradient elution table for chromatographic separation.

Time (min)	Flow Rate (mL/min)	% Mobile Phase B (v/v)	Curve^†
Initial	0.4	2.5	Initial
0.6	0.4	2.5	6 (linear)
2.8	0.4	35	6 (linear)
4.5	0.4	80	5 (concave)
5.5	0.4	2.5	1 (step)

^†

For the gradient curve algorithm refer to www.waters.com