Skip to main content
. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Mar 2;1083:86–92. doi: 10.1016/j.jchromb.2018.02.043

Table 1.

Gradient elution table for chromatographic separation. Mobile phases A and B are 0.1% (v/v) HFBA (aq.) and 0.1% HFBA (v/v) in methanol, respectively.

Time (min) Flow Rate (mL/min) % Mobile Phase B (by volume)
Initial 0.2 5
0.75 0.5 5
1.75 0.5 50
3.25 0.3 95
3.5 0.3 5
5.0 0.2 5