. Author manuscript; available in PMC: 2021 Mar 1.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Mar 2;1083:86–92. doi: 10.1016/j.jchromb.2018.02.043

Table 5.

Comparison of calibration curve slopes for different types of matrices. The linear calibration range used for all matrices was identical (please refer Table 6 for the range)

Analyte	0.1% HFBA (aq.) Matrix	Urine Pool Matrix		Synthetic Urine Matrix
Analyte	Slope	Slope	Difference (%)^*	Slope	Difference (%)^*
HDA	0.244	0.238	2.49	0.238	2.49
IPDA	1.818	1.877	3.19	1.741	4.33
BMAA	0.302	0.292	3.37	0.303	0.33
TMAO	0.060	NR^†	NR^†	0.059	1.68

Absolute percent differences relative to 0.1% HFBA (aq.) matrix

^†

NR = Not reported because of high endogenous TMAO content in a urine pool