Figure 1.

Poor deletion efficiency of paired sgRNAs and CRISPR-deletion (CRISPR-del) model. (A) Model for CRISPR-del and its improvement by inhibition of nonhomologous end-joining (NHEJ). (B) Reanalysis of significant growth-promoting gene “hits” identified in the CRISPR-del negative selection screen in Huh7 cells from Zhu et al. (2016). Each hit is targeted by several individual paired sgRNAs. Histogram displays the percentage of paired sgRNAs that effectively perturb their target gene. The dashed line represents the median (42.9%). (C) Scatterplot displays paired sgRNAs targeting gene hits from B. The x-axis indicates the mean of the two sgRNAs predicted on-target efficiency (calculated with the Rule Set II scoring algorithm from Doench et al. 2016); y-axis, log₂-transformed fold-change in paired sgRNA detection after cell passaging (growth phenotype). R indicates Pearson's correlation; P, P-value.