Figure 3.

Histone H3K56 acetylation does not account for Rtt109-dependent replication slowdown. (A) Replicon length is invariant to H3K56-related perturbations: Shown are the replicon lengths of the indicated substitution and deletion mutants. (B) H3K56 mutation does not increase fork velocity: Shown are the fork velocity and initiation frequency of the indicated mutants, as defined from the DNA content of cells progressing synchronously through S phase (for details, see Supplemental Fig. S2). (C–F) H3K56 mutation does not promote Pol2 progression: same as Figure 2, F through H, for the indicated mutants.