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Detection of the cell competence to undergo the cell cycle progression in Arabidopsis shoot-derived protoplasts. 5-Ethynyl-2´-deoxyuridine (EdU) was added to the cell suspension at 12 h after isolation. The cells were fixed at 36 and 60 h after isolation, and the nuclei were isolated and subjected to the standard procedure for EdU detection. Nuclei are red; EdU stained nuclei are in green. White arrow marked positive nuclei, while magenta arrow— negative. Scale bar—20 µm.
