Table 1.
Composition of different nutrients media (in mg/L) for in vitro growth of Arabidopsis seedlings (TK). Arabidopsis medium (AM; half-strength Murashige and Skoog Medium) [15] and for the optimized medium described in [9] (TK1).
| Compound | AM (½ MS) | TK1 |
|---|---|---|
| Macronutrients (mg/L) | ||
| NH4NO3 | 825 | - |
| KNO3 | 950 | 900 |
| NH4H2PO4 | - | 230 |
| MgSO4·7H2O | 180 | 200 |
| KH2PO4 | 68 | - |
| Ca(NO3)2 4H2O | - | 250 |
| CaCl2 2H2O | 220 | - |
| Fe-Chelate (*) | 2.5 ml | 2 ml |
| Micronutrients | ½ micro MS | ½ micro TK |
| Organic compounds (mg/L) | ||
| Bacto-tryptone | - | 150 |
| Vitamines (**) | ½ B5 vitamines | ½ B5 vitamines |
| Myoinositol | 50 | 50 |
| Sucrose | 10000 | 10000 |
| MES | 200 | 200 |
| pH | 5.6 | 5.6 |
| (a). Micronutrient concentrations in the medium (***). | ||
| Micronutrients (mg/L) | micro MS | micro TK |
| H3BO3 | 6.2 | 4 |
| MnCl2 4H2O | 16.8 | 18 |
| ZnSO4 7H2O | 30 | 1.8 |
| Na2MoO4 2H2O | 0.25 | 0.25 |
| NaI | 0.76 | 0.8 |
| CuSO4 5H2O | 0.025 | 0.2 |
| CoCl2 6H2O | 0.025 | 0.2 |
* Fe-chelate—20 mM stock solution. ** B5 vitamins: 10 mg/L B1; 1 mg/L- B6 and 1 mg/l PP (prepared as 1000 × stock solution). *** Micronutrients stock has been prepared separately (prepared as 1000 × stock solution).