REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-GFAP	Cell Signaling Technology	Cat#3670; RRID: AB_561049
Mouse anti-NeuN	Millipore	Cat#MAB377; RRID: AB_2298772
Biotinylated horse anti-mouse	Vector Laboratories	Cat#BA-2000; RRID: AB_2313581
Texas Red streptavidin	Vector Laboratories	Cat#SA-1200; RRID: AB_2336464
Chicken anti-beta galactosidase	Abcam	Cat#ab9361; RRID: AB_307210
Rabbit anti-S100β	Abcam	Cat#ab52642; RRID: AB_882426
Goat anti-chicken 488	Abcam	Cat#ab150169; RRID: AB_2636803
Donkey anti-rabbit 594	Abcam	Cat#ab150076; RRID: AB_2782993
Goat anti-mouse 594	Abcam	Cat#A-21125; RRID: AB_2535767
Bacterial and Virus Strains
AAV5.GfaABC1D.cytoGCaMP6f.SV40	Penn Vector Core	Cat#AV-5–52925; Custom preparation
Chemicals, Peptides, and Recombinant Proteins
Tamoxifen	Sigma-Aldrich	Cat#T5648; CAS: 10540–29-1
Sunflower oil	Sigma-Aldrich	Cat#S5007; CAS: 8001–21-6
Methoxamine hydrochloride	Sigma-Aldrich	Cat#M6524; CAS: 61–16-5
Experimental Models: Organisms/Strains
Mouse: C57Bl/6J	The Jackson Laboratory	JAX: 000664; RRID: IMSR_JAX:000664
Mouse: B6.Cg-Tg(GFAP-cre/ERT2)505Fmv/J	The Jackson Laboratory	JAX: 012849; RRID: IMSR_JAX:012849
Mouse: B6(Cg)-Stim1tm1Rao/J	The Jackson Laboratory	JAX: 023350; RRID: IMSR_JAX:023350
Mouse: FVB.129S4(B6)-Gt(ROSA)26Sortm1Sor/J	The Jackson Laboratory	JAX: 009427: RRID: IMSR_JAX:009427
Software and Algorithms
Sirenia Acquisition v1.7.7	Pinnacle Technologies	https://www.pinnaclet.com/
nVista HD v2.0.4	Inscopix	https://inscopix.com
Data Processing Software v1.2.1	Inscopix	https://inscopix.com
OpenEx	Tucker-Davis Technologies	https://www.tdt.com/
Prairie View	Bruker	https://www.bruker.com/
ImageJ	[69]	https://imagej.nih.gov/ij/
Fiji	[70]	https://imagej.net/Fiji
VitalRecorder v3,0,0,0	Kissei Comtec Co., LTD	http://www.sleepsign.com/
SleepSign for Animal v3,0,0,812	Kissei Comtec Co., LTD	http://www.sleepsign.com/
Running Wheel Manager Data Acquisition Software	Med Associates Inc.	Cat#SOF-860; https://www.med-associates.com/
VitalView Telemetry Data Acquisition Software	Starr Life Sciences Corp.	https://www.starrlifesciences.com/
Astrocyte Quantitative Analysis	[28]	https://github.com/yu-lab-vt/aqua
SigmaPlot v11.0	Systat Software, Inc	https://systatsoftware.com/products/sigmaplot/
SPSS for Windows 25	IBM	https://www.ibm.com/analytics/academic-statistics-software
R v3.6.0	[71]	https://www.r-project.org/
Aligning and processing algorithm	This paper	Available upon request