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. 2021 Feb 26;9(2):e001696. doi: 10.1136/jitc-2020-001696

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See https://creativecommons.org/licenses/by/4.0/.

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Prognostic significance of HDAC1, HDAC2, and HDAC3 expression in STS patients. (A) Prognostic significance of HDAC1, HDAC2, HDAC3 mRNA expression in 263 TCGA sarcoma samples. HDAC1, HDAC2, and HDAC3 mRNA upregulation is significantly correlated with poor overall survival (p<0.001); the mRNA expression levels calculated by RSEM (TCGA V2) were scaled as z-scores with threshold ±2.0. (B) Prognostic significance of HDAC1, HDAC2, and HDAC3 mRNA expression in 49 patients with STS. The mRNA expression in STS was detected using real-time PCR. Kaplan-Meier and log rank analyses for overall survival of STS patients with available clinical follow-up data identified two subgroups with higher or lower expression. HDAC1 and HDAC2 mRNA expression is significantly associated with overall survival (p=0.035 and p=0.012, respectively), and HDAC3 is not much significantly associated with overall survival (p=0.147). (C) Western blot analysis of whole cell lysates derived from HT-1080 cells after class I type HDACs siRNA transfection. (D) The apoptosis rate of HT-1080 and SK-LMS-1 after transfected with class I type HDACs (HDAC1, HDAC2 and HDAC3). (E-F) Flow cytometry analysis of surface levels of PD-L1 on class I type HDACs (HDAC1, HDAC2 and HDAC3) knockdown HT-1080 cells (left) and SK-LMS-1 cells (right) and quantification. Data are mean±SD of three independent experiments. *P<0.01 (two-tailed unpaired t-test). HDAC, histone deacetylase; STS, soft tissue sarcoma; TCGA, The Cancer Genome Atlas.