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(A) CaSki 3D spheroids do not respond to radiation. (B) CaSki 3D spheroids respond to Cisplatin. (C) CaSki 3D spheroids respond to 2-DG. (D) 2-DG treatment has stronger effects than Cisplatin in reducing the sizes of the CaSki 3D organoids. (E) 2-DG treatment downregulates mesenchymal and upregulates epithelial gene expression of CaSki cells. Experiments were repeated 3–6 times.
