Figure 1. Ad4-H5-Vtn shedding at mucosal sites.

(A) qPCR was performed on the nasal, tonsillar, and rectal swabs for the detection of viral shedding up to 4 weeks (28 days) after vaccination. Immunization is shown for intranasal (red, n = 25), tonsillar (blue, n = 28), and oral (black, n = 10) routes. (B) Recovery of infectious virus (green) on nasal swabs was measured in comparison with qPCR (gray) in vaccinees immunized by the intranasal route (left). The distribution of days of shedding (middle) and the titers of recovered virus over time (right) are shown (n = 25). The red horizontal bar indicates the median value.