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. 2021 Mar 1;131(5):e140853. doi: 10.1172/JCI140853

Figure 4. Filaria-associated lymphatic pathology is dependent on IL-4 receptor immune responses.

Figure 4

(A) Representative images, (B) quantified aberrant lymphatics, and (C) quantified hind-limb ICG dye retention in WT and IL-4Rα–/– BALB/c and C57BL/6J mice, 14 dpi (n = 20 WT BALB/c sham, n = 21 WT BALB/c BmL3, n = 8 IL-4Rα–/– BALB/c sham, n = 15 IL-4Rα–/– BALB/c BmL3, n = 10 WT C57BL/6J sham, n = 10 WT C57BL/6J BmL3, n = 5 IL-4Rα–/– sham and IL-4Rα–/– BmL3). (D) Evan’s blue left-hind-limb dermal retention in WT and IL-4Rα–/–mice, 14 dpi (n = 8 WT BALB/c BmL3, n = 5 IL-4Rα–/– BALB/c BmL3, n = 8 WT C57BL/6J BmL3, n = 5 IL-4Rα–/– sham and IL-4Rα–/– BmL3). (E) Comparison of circulating levels of lymphangiogenic molecules between WT C57BL/6J WT and IL-4Rα–/– BmL3-infected mice, 14 dpi. Heatmap plots median fold-change in analyte from sham-infected mouse group; red = fold-increase from sham-infected, blue = fold-decrease (n = 6 WT BmL3, n = 7 IL-4Rα–/– BmL3). (F) Plots of lymphangiogenic analytes achieving statistical significance. Histograms show the mean ± SEM. Data were pooled from 2–3 individual experiments. *P < 0.05, ***P < 0.001 by 1-way ANOVA with Tukey’s multiple-comparison post hoc test (B–D) or 2-tailed Student’s t test between marked groups (F).