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. 2021 Mar 1;131(5):e143173. doi: 10.1172/JCI143173

Figure 1. RagA/B cKO mice exhibit severe hypertrophy reminiscent of LSD.

Figure 1

(A) Representative images of control (Ctr) and RagA/B cKO mouse hearts at 12 weeks old. (B) Quantitative analysis of total HW/TL at 12 weeks. n = 6. (C) Left: representative micrographs of WGA staining of the LV. Right: quantitative analysis of CSA at 12 weeks. n = 6. (D) Relative ANF and BNP mRNA expression at 12 weeks. n = 7. (E) Representative immunoblots of 1.5-month old control and RagA/B cKO mouse heart homogenates. Mice were treated with CQ or vehicle for 4 hours before euthanasia. Quantitative analyses of LC3-II/α-tubulin are shown. n = 4. (F) Representative echocardiographic tracings of control and RagA/B cKO mouse hearts at 12 weeks. Quantitative analyses of FS and LVEDD are shown. n = 6. (G) Kaplan-Meier survival curves. Results are expressed as mean ± SEM in B, C, E, and F. In D, results are shown as box plots, showing the median (center line) and IQR. Whiskers represent minima and maxima within 1.5 IQR as indicated. *P < 0.05; **P < 0.01, ANOVA.