Table 2.

The effects of different parameters on Trolox equivalent antioxidant capacity (TEAC) and total phenolic content (TPC) outcomes during optimization of Brazilian red propolis ethanolic extracts.

Term	SS	df	MS	F-Value	p-Value	Remarks
TEAC
Model	1,247,423.00	9	138,602.55	603.14	<0.0001	significant
${\mathrm{X}}_1$	8019.54	1	8019.54	34.89	0.0183
${\mathrm{X}}_2$	74,849.67	1	74,849.67	325.63	0.0020
${\mathrm{X}}_3$	425,485	1	425,484.7	1851.06	0.0003
${\mathrm{X}}_1^2$	83.58	1	83.58	0.3636	0.5347
${\mathrm{X}}_2^2$	144,174	1	144,173.8	627.22	0.0010
${\mathrm{X}}_3^2$	530,460	1	530,460.1	2307.80	0.0002
${\mathrm{X}}_1{\mathrm{X}}_2$	1153.35	1	1153.35	5.02	0.1092
${\mathrm{X}}_1{\mathrm{X}}_3$	1.87	1	1.87	0.0081	0.9254
${\mathrm{X}}_2{\mathrm{X}}_3$	94,179.02	1	94,179.02	409.73	0.0016
Residual	1149.29	5	229.86
Lack of Fit	846.99	3	282.33	1.87	0.3673	non-significant
Pure error	302.30	2	151.15
Total	1,248,572	14
R2	0.9991
Adj-R2	0.9974
TPC
Model	627.04	9	69.67	34.75	0.0006	significant
${\mathrm{X}}_1$	134.88	1	134.88	67.27	0.0004
${\mathrm{X}}_2$	10.38	1	10.38	5.18	0.0719
${\mathrm{X}}_3$	9.45	1	9.45	4.71	0.0820
${\mathrm{X}}_1^2$	39.29	1	39.29	19.60	0.0068
${\mathrm{X}}_2^2$	250.65	1	250.65	125.01	<0.0001
${\mathrm{X}}_3^2$	81.65	1	81.65	40.72	0.0014
${\mathrm{X}}_1{\mathrm{X}}_2$	5.97	1	5.97	2.98	0.1449
${\mathrm{X}}_1{\mathrm{X}}_3$	1.10	1	1.10	0.5473	0.4927
${\mathrm{X}}_2{\mathrm{X}}_3$	132.72	1	132.72	66.20	0.0005
Residual	10.02	5	2.00
Lack of Fit	1.78	3	0.5938	0.1441	0.9251	non-significant
Pure error	8.24	2	4.12
Total	637.07	14
R2	0.9843
Adj-R2	0.9559

X₁: Time (min), X₂: Temperature (°C), X₃: EtOH (%), SS: Sum of squares, DF: Degree of freedom, MS: Mean square, R²: Quadratic correlation coefficient; Adj-R²: Adjusted quadratic correlation coefficient. TEAC: Trolox equivalent antioxidant capacity; TPC: Total phenolic content.