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. 2021 Feb 16;22(4):1937. doi: 10.3390/ijms22041937

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Neuromuscular junction integrity changes with lifelong Ulk1 deficiency. (A) Average number of innervated and denervated neuromuscular junctions (NMJs) in the diaphragm of MKO and LM mice (p = 0.0227). Data are presented as mean ± SD. * = significantly different from LM. (B) Representative images of the pre- (red) and post-synaptic (green) terminals of the NMJs from both MKO and LM diaphragm muscle sections. The merged image depicts both innervated (yellow) and denervated (green) NMJs. Innervation was defined as the overlap of pre- and post-synaptic structures, while the complete or partial absence of the presynaptic terminal classified the NMJ as denervated.