Figure 5.

Lifelong Ulk1 deficiency results in an accumulation of dysfunctional mitochondria. (A) Mitochondrial content assessed through citrate synthase enzyme kinetic rates (p = 0.0443). (B) Mitochondrial oxygen consumption during Leak, State III, cytochrome C (to check for intact mitochondrial membrane), and uncoupled respiration normalized to wet tissue weight. (p = 0.026) (C) Mitochondrial oxygen consumption during Leak, State III, and uncoupled respiration normalized to citrate synthase activity. (p = 0.041, p = 0.002, p < 0.001, respectively) (D) Percent ROS production normalized to oxygen consumption during leak respiration to account for differences in oxygen flux (p = 0.0428). (E) Mitochondrial DNA content normalized to nuclear DNA content (p = 0.0309). (F) Representative oxygen flux measurements during a mitochondrial respiration protocol (O2k Oroboros). All data are presented as mean ± SD. * = significantly different from LM.