Table 1.

Skeletal muscles collected postmortem, assays performed, and tissue function.

Tissue	Assay	Function
Extensor digitorum longus	In vitro contractile properties	Toe extension and foot dorsiflexion
Soleus	In vitro contractile properties	Foot plantarflexion
Tibialis anterior	In vivo contractile properties Autophagy flux (Chloroquine Treatment) Autophagy flux (multi-photon) MyHC isoform distribution (IHC) Fiber cross-sectional area (IHC) Fiber central nuclei (IHC)	Foot dorsiflexion
Gastrocnemius	Mitochondrial content (citrate synthase) Mitochondrial respiration mtDNA damage (PCR) Autophagy protein content (WB) Mitochondrial remodeling protein content (WB)	Foot plantarflexion
Diagram	Neuromuscular junction (IHC)	Ventilation

MyHC, myosin heavy chain; IHC, immunohistochemistry; WB, Western blot; PCR, polymerase chain reaction.