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. 2021 Mar;191(3):575–583. doi: 10.1016/j.ajpath.2020.12.010

Figure 4.

Figure 4

In vivo two-photon (2P) imaging of parenchymal movement of brain-implanted Raji cells in regions with and without blood-brain barrier (BBB) leakage. A: In vivo 2P time-lapse images, showing tracking of intracortical movement of Raji cells in Raji cell–implanted mice over 45 minutes. White tracks indicate cells showing movement over the 45-minute imaging interval. B: Quantification of traveled distance measured over 45 minutes, shown in A. Data are shown for individual cells (five cells per mouse) and as averages of five cells per mouse. C: Lymphoma cell adhesion assay. Quantification of adhered lymphoma cells labeled with Calcein-AM on different coating conditions: uncoated, fibrinogen, and albumin. D: Quantification of adhered lymphoma cells to coated plasma isolated from wild-type and Fga–/– mice. Data are given as means ± SEM (C and D). n = 3 mice with BBB leakage and without BBB leakage (B); n = 3 independent experiments (C and D). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001 (two-way analysis of variance with the Tukey multiple-comparisons test). Scale bar = 25 μm (A). Ex/em, excitation/emission.