Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 6:eabf4063. doi: 10.1126/science.abf4063

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).

This is an open-access article distributed under the terms of the Creative Commons Attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 2 — (A) Example flow cytometry plots showing staining patterns of SARS-CoV-2 antigen probes on memory B cells (See fig. S1 for gating). One unexposed donor and three convalescent COVID-19 subjects are shown. Numbers indicate percentages. (B) Gating strategies to define IgM⁺, IgG⁺, or IgA⁺ SARS-CoV-2 Spike-specific memory B cells. The same gating strategies were used for RBD- or Nucleocapsid-specific B cells. (C) Cross-sectional analysis of frequency (% of CD19⁺ CD20⁺ B cells) of SARS-CoV-2 S-specific total (IgG⁺, IgM⁺, or IgA⁺) memory B cells. Pseudo-first order kinetic model for best fit curve (R = 0.38). (D) Longitudinal analysis of SARS-CoV-2 Spike-specific memory B cells. (E) Cross-sectional analysis of SARS-CoV-2 RBD-specific total (IgG⁺, IgM⁺, or IgA⁺) memory B cells. Second order polynomial model for best fit curve (R = 0.46). (F) Longitudinal analysis of SARS-CoV-2 RBD-specific memory B cells. (G) Cross-sectional analysis of SARS-CoV-2 Nucleocapsid-specific total (IgG⁺, IgM⁺, or IgA⁺) memory B cells. Pseudo-first order kinetic model for best fit curve (R = 0.44). (H) Longitudinal analysis of IgG⁺ SARS-CoV-2 Nucleocapsid-specific memory B cells. (I) Cross-sectional analysis of SARS-CoV-2 Spike-specific IgG⁺ memory B cells. Pseudo-first order kinetic model for best fit curve (R = 0.49). (J) Longitudinal analysis of SARS-CoV-2 Spike-specific IgG⁺ memory B cells. (K) Cross-sectional analysis of SARS-CoV-2 Spike-specific IgA⁺ memory B cells. Second order polynomial model for best fit curve (|R| = 0.32). (L) Longitudinal analysis of SARS-CoV-2 Spike-specific IgA⁺ memory B cells. (M) Cross-sectional analysis of SARS-CoV-2 Spike-specific IgM⁺ memory B cells. Second order polynomial model for best fit curve (|R| = 0.41). (N) Longitudinal analysis of SARS-CoV-2 Spike-specific IgM⁺ memory B cells. (O) Fraction of SARS-CoV-2 antigen-specific memory B cells that belong to indicated Ig isotypes at 1-8 months PSO. Mean ± SEM. (P) Cross-sectional analysis of SARS-CoV-2 RBD-specific IgG⁺ memory B cells. Second order polynomial model for best fit curve (|R| = 0.51). (Q) Cross-sectional analysis of SARS-CoV-2 Nucleocapsid-specific IgG⁺ memory B cells. Second order polynomial model for best fit curve (|R| = 0.51). n = 20 unexposed subjects (gray circles) and n = 160 COVID-19 subjects (n = 197 data points, white circles) for cross-sectional analysis. n = 36 COVID-19 subjects (n = 73 data points, white circles) for longitudinal analysis. The dotted black line indicates limit of detection (LOD). The dotted green line indicates limit of sensitivity (LOS).