Skip to main content
. 2021 Jan 6:eabf4063. doi: 10.1126/science.abf4063

Fig. 3. SARS-CoV-2 circulating memory CD8+ T cells.

Fig. 3

(A) Representative flow cytometry plots of SARS-CoV-2-specific CD8+ T cells (CD69+ CD137+, See fig. S3 for gating) after overnight stimulation with S, N, M, ORF3a, or nsp3 peptide pools, compared to negative control (DMSO). (B) Cross-sectional analysis of frequency (% of CD8+ T cells) of total SARS-CoV-2-specific CD8+ T cells. Continuous decay preferred fit model, t1/2 = 125 days. R = -0.24, p = 0.0003. (C) Longitudinal analysis of total SARS-CoV-2-specific CD8+ T cells in paired samples. (D) Cross-sectional analysis of Spike-specific CD8+ T cells. Linear decay preferred model, t1/2 = 225 days. R = -0.18, p = 0.007. (E) Longitudinal analysis of Spike-specific CD8+ T cells in paired samples. (F, G) Distribution of central memory (TCM), effector memory (TEM), and terminally differentiated effector memory cells (TEMRA) among total SARS-CoV-2-specific CD8+ T cells. n = 169 COVID-19 subjects (n = 215 data points, white circles) for cross-sectional analysis. n = 37 COVID-19 subjects (n = 83 data points, white circles) for longitudinal analysis. The dotted black line indicates limit of detection (LOD). The dotted green line indicates limit of sensitivity (LOS).