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. 2021 Feb 26;59(1):230–239. doi: 10.1080/13880209.2021.1883678

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Andro enhanced on the expression of Nrf2 and Keap1 proteins and mRNA in PC12 cells induced by Al(mal)₃. Cells were incubated with 700 μM Al(mal)₃ and 5 or 10 μM Andro for 24 h. The expression of Nrf2 and Keap1 were detected by western blot, GAPDH was used as loading control (A); Quantitative analysis of Nrf2 (B) and Keap1 (C) protein expression levels; The levels of Nrf2 and Keap1 (D) mRNA were analysed using RT-qPCR. *p < 0.05, **p < 0.01 versus the control, ^#p < 0.05, ^##p < 0.01 versus Al(mal)₃ group was considered statistically significant differences (n = 3).