Figure 4.
Andro increased the nuclear expression of Nrf2 in Al(mal)3-induced PC12 cells. Cells were co-incubated with 700 μM Al(mal)3 and 10 μM Andro for 24 h. The fluorescence localization of Nrf2 was measured with immunofluorescence. An anti-Nrf2 antibody was used to detect Nrf2 localization using a fluorescence microscope. Green colour represented FITC-positive Nrf2, Blue colour represented DAPI-positive nucleus. Bar = 100 μm (A); Quantitative immunofluorescence analysis of Nrf2 in cytoplasm and the nucleus (B). *p < 0.05, **p < 0.01 versus the control, ##p < 0.01 versus Al(mal)3 group was considered statistically significant differences (n = 6).