Figure 7.
Effect of Andro on expression and location of keap1 protein in PC12 cells induced by Al(mal)3. Cells were incubated with 700 μM Al(mal)3 and 10 μM Andro for 24 h. The fluorescence localization of keap1 was measured with immunofluorescence. An anti-keap1 antibody was used to detect keap1 localization using a fluorescence microscope. Red colour represented Cy3-positive keap1, Blue colour represented DAPI-positive nucleus. Bar = 100 μm (A); Quantitative immunofluorescence analysis of Keap1 in cytoplasm (B). *p < 0.05 versus the control, #p < 0.05 versus Al(mal)3 group was considered statistically significant differences (n = 6).