Figure 8.
Effect of silencing p62 or Nrf2 on the expression of p62, LC3, Nrf2 proteins and mRNA in PC12 cells co-treated with Al(mal)3 and Andro. After transfection with Nrf2 or p62 siRNA, cells were incubated with 700 μM Al(mal)3 and 10 μM Andro for 24 h. The expression of p62 (A), LC3 (B) and Nrf2 (E) were detected by western blot, GAPDH was used as loading control. The levels of p62 (B), LC3 (C) and Nrf2 (F) mRNA were analysed using RT-qPCR. #p < 0.05, ##p < 0.01 versus the control, *p < 0.05, **p < 0.01 versus Al(mal)3 + Andro group was considered statistically significant differences (n = 3).