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. 2021 Feb 24;8(1):1891725. doi: 10.1080/20018525.2021.1891725

Table 3.

Summary of studies investigating the association between FeNO and loss of asthma control in adults

Loss of control     Association of FeNO
Tsurikisawa et al 2012 [51] Prospective 2-year study 90 patients with moderate or severe asthma on ICS and without clinical symptoms for ≥6 months.
Mean (SD) age: 49.1 (14.6) years in exacerbation-free group (n = 50), 50.9 (15.9) years in exacerbation group (n = 40).
Mean (SD) FeNO: 25.6 (12.0) ppb in exacerbation-free group, 43.4 (27.3) ppb in exacerbation group.
Multivariate logistic regression showed a rank order of predictors of successful ICS reduction while retaining asthma control (exacerbations): acetylcholine PC20 (p < 0.01); length of time with no clinical symptoms before ICS reduction (p < 0.01); FeNO (p = 0.028); and FEV1 (% predicted) (p = 0.03).
Jones et al 2001 [52] Prospective 6-week study 78 patients with mild to moderate asthma on ICS for ≥6 months.
Mean age 42.9 (range 18–74) years.
Mean FeNO 9.38 (95% CI, 2.72; 32.35) ppb.
By regression analysis, single measurements and different FeNO cut-off values (>10 ppb, >15 ppb, or an increase of >60% above baseline) had positive predictive values ranging from 80 to 90% for predicting loss of control.
Matsunaga et al 2012 [53] Prospective observational 12-week study 250 patients with stable asthma on ICS with/without LABA and/or other therapies.
Mean (SD) age 46.6 (14.7) years.
Mean (SD) FeNO 34.6 (22.0) ppb.
Multivariate logistic regression analysis showed that a FeNO level >39.5 ppb gave 67% sensitivity and 76% specificity for identifying the patients with poorly controlled asthma.
Michils et al 2008 [54] Prospective longitudinal 3-month study 341 patients with mild, moderate or severe asthma, ICS naïve, or on ICS with/without LABA and/or other therapies.
Mean (SD) age 41 (16) years.
Geometric mean (range) FeNO 32.9 (13.8–78.1) ppb.
In the whole population, FeNO >45 ppb was not associated with well-controlled asthma (negative predictive value 88%), p < 0.001. The use of FeNO for predicting asthma control was less effective as ICS dose increased.
Ozier et al 2011 [55] Prospective 6-month study 90 patients with severe or non-severe asthma, 72% on ICS, 50% on LABA and 6.7% on OCS.
Mean (SD) age: 38.5 (2.1) years in controlled group (n = 62), 44.8 (3.2) years in uncontrolled group (n = 28).
Mean baseline FeNO not stated.
A correlation analysis was used to determine that a low baseline FeNO <31 ppb was a good predictor of not losing good asthma control (negative predictive value ≥95%), but high FeNO >31 ppb was only a modest predictor of loss of asthma control (positive predictive value 67%).
Yamashita et al 2016 [56] Retrospective 1-year study 71 patients with mild asthma newly diagnosed and not on ICS. 37 patients continued clinic visits for 1 year.
Mean (SD) age 50.9 (15.6) years.
Mean (SD) FeNO 42.2 (31.9) ppb.
In a multivariate logistic regression, FeNO levels at the first visit were identified as a possible predictor of asthma control (OR 0.9459 (95% CI, 0.9024; 0.9915); p = 0.021).
Tsilogianni et al 2016 [57] Ongoing cohort 6-month study 170 patients with mild to moderate (72%) or severe (28%) asthma on ICS and/or LABA, LTRA, OCS or omalizumab.
Median (range) age 53 (42–62) years.
Median (range) FeNO 22 (16–35) ppb.
FeNO levels differed significantly between patients with well-controlled asthma and those with uncontrolled asthma, median (IQR) 21 (14–28) vs. 45 (19–67), p < 0.001. Receiver operating characteristic analysis for the whole study population showed that FeNO <43 ppb was only a modest predictor of well-controlled asthma (negative predictive value 50%).
      No effect of FeNO
Kilic et al [58] Cross-sectional outpatient study 81 women (41 obese) with mild (84%), moderate (12%) or severe (4%) stable asthma on ≥1 asthma therapy for ≥6 months.
Mean (SD) age: 53.9 (10.2) in obese group, 47.3 (15.7) in nonobese group.
Median (range) FeNO: 21.0 (11.0–52.0) in obese group, 22.5 (6.0–297.0) in nonobese group.
By regression analysis, high BMI was found to be the only significant factor that contributed to poor asthma control, with no effect of FeNO (p = 0.799 in the obese group, p = 0.194 in the nonobese group).

BMI, body mass index; CI, confidence interval; FeNO, fractional exhaled nitric oxide; FEV1, forced expiratory volume in 1 second; ICS, inhaled corticosteroids; IQR, interquartile range; LABA, long-acting beta agonist; LTRA, leukotriene receptor antagonists; n, number of patients; PC20, provocative dose causing a 20% fall in FEV1; OR, odds ratio; ppb, parts per billion; SD, standard deviation.