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NRK-52E cells incubated with TGF-β1 mediate recipient cardiomyocyte hypertrophy. (A) Electron microscopy image of NRK-52E-derived MVs, showing a size of approximately 60 to 80 nm in diameter. ×10k, Scale bar: 100 nm (n = 5). (B) The size of cardiomyocyte was measured with or without the incubation of MVs. (C) Protein concentration was measured with or without the incubation of MVs. (D) ANP content was detected with or without the incubation of MVs. *p < 0.05 versus control.
