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. 2021 Feb 17;12:621039. doi: 10.3389/fimmu.2021.621039

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Copyright © 2021 Parikh, Malvar, Shapiro, Turman, Song, Alberton, Lococo, Mejia-Vilet, Madhavan, Zhang, Yu, Satoskar, Birmingham, Jarjour, Rovin and Ganesan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fc receptor gamma chain is highly expressed in the patient's kidney with LN, and its expression is confined to the periglomerular (PG) region. Three color confocal IF image that is representative of 5 human LN kidney showing the expression of FcRγ (bottom row) in the PG region of the kidney and no expression in the representative of the three HC kidneys (top row). The third column shows merged images of the first 2 columns, i.e., synaptopodin (podocyte marker in green) was used as a landmark for G in the first column, FcRγ (red) in the second column plus differential interference contrast (DIC) microscopy and DAPI staining of nuclei (blue). The fourth column shows the zoomed image of the third column highlighted using a dotted square. The scale bar in the second column represents 20 μm.