Figure 4.

Fc receptor gamma chains colocalize with dendritic cell markers CD11c and MHCII in human LN kidney. Three color IF images that are representative of the 3 human LN kidneys showing colocalization of CD11c (labeled using anti-CD11c antibody followed by Alexa-488 dye conjugated secondary antibody that shows green emission color in confocal microscopy (green) (top row) with FcRγ labeled using anti-FcRγ antibody followed by Alexa-594 conjugated secondary antibody that shows red emission color in confocal microscopy (red). The bottom row shows the colocalization of MHC II labeled using anti-CD11c antibody followed by Alexa-488 dye conjugated secondary antibody that shows green emission color in confocal microscopy (green) with FcRγ labeled using anti- FcRγ antibody followed by Alexa-594 conjugated secondary antibody that shows red emission color in confocal microscopy (red). The third column shows the colocalization of red and green as yellow in the first 2 columns along with DIC and DAPI staining of nuclei (blue). The fourth column shows the zoomed image of the third column highlighted using a dotted square.