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. 2021 Feb 17;12:621039. doi: 10.3389/fimmu.2021.621039

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Copyright © 2021 Parikh, Malvar, Shapiro, Turman, Song, Alberton, Lococo, Mejia-Vilet, Madhavan, Zhang, Yu, Satoskar, Birmingham, Jarjour, Rovin and Ganesan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fc receptor gamma chain does not colocalize with plasmacytoid DC marker CD123, but colocalizes with monocyte-derived DC marker SIRPA and DC-SIGN in the human LN kidney. Three color confocal IF images that are representative of 3 human LN kidneys showing no colocalization of CD123 (green) with Fc receptor gamma chain (FcRγ) (red) (top row) in the TI region of the kidney, but colocalize with monocyte-derived DC cell marker SIRPα (green) (middle row) and DC-SIGN (green) with FcRγ (red) (bottom row) in the PG region. The third column shows merged images of the first 2 columns plus DIC microscopy and DAPI staining of nuclei (blue). The fourth column shows the zoomed image of the third column highlighted using a dotted square.