Figure 6.

Fc receptor gamma chain colocalizes with previously identified circulating inflammatory dendritic cell (infDC) marker CD163 and CD163 expression is overexpressed in human LN compared to HCs. (A) Three color IF images that are representative of 3 human LN kidneys showing colocalization of CD163 in green (first) with FcRγ in red (middle) in the PG region. The second row shows a zoomed portion of images of the top row highlighted in the dotted square. The third column shows the merging of the first 2 along with DIC and DAPI staining of nuclei (blue). (B) Three color IF images that are representative of the 3 human LN kidneys showing colocalization of CD163 in green (first) with FcRγ in red (middle) in the tubulointerstitial (TI) region. (C) The bar graph portrays the quantification of infDCs based on CD163 expression using green color from anti-CD163 staining from 18 G images of 3 different HCs and 16 glomeruli images from 4 different LN samples using ImageJ software. The area alone (graph in left) and area × mean fluorescence intensity (graph in left) of CD163 (green) were measured and plotted with mean ± SD. The data were analyzed by unpaired t-test (one-tailed) using each measurement, and p-values were indicated in the bar graph.