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. 2021 Mar 1;9:15. doi: 10.1186/s40364-021-00265-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Unique CD4⁺ subsets revealed by scRNA-seq analysis. a UMAP plot of CD69^highCD4⁺ population and CD69^lowCD4⁺ population. These 2515 cells can be divided into 4 subsets. b Heatmap showing average expression level of cell-type genes expressed by the 4 clusters. c Histogram showed the fractions of different cell-type in CD69^highCD4⁺ population and CD69^lowCD4⁺ population for each AML patient and healthy donors’ BM cells, colored based on cell type. d The branched trajectory of state transition of naïve CD4⁺ T cells, TH17-like cells, and Treg cells in a two-dimensional state-space inferred by Monocle (version 2.14.0). Each dot corresponded to one single cell, colored according to its cluster label. e Expression maps showing log-normalized expression of typical markers (IL7R, IL2RA, MKI67, KLRB1, FOXP3, and CTLA4) in the differentiation of Naïve CD4⁺ T to TH17-like cells and/or Treg cells. Data are shown as log-normalized expression. Yellow indicates high expression, dark blue indicates low expression. f Violin plot showing the expression levels of functional genes (IGF1R, RORC, KLRB1, AHNAK, and IL10RA)