Table 1.
Immune cell types and functions in AML BM microenvironment
| Cluster | Subset | Feature genes | Anti-tumor or immunosuppressive /dysfunctional |
|---|---|---|---|
| Mast cell | – | High: KIT, GATA2, LMO4, CPA3, TPSAB1, TPSB2 | Anti-tumor |
| DC | pDC | High: CD36, FLT3, LY86, PTPRCAP, GLIPR2, IL3RA, SEMA4D, JCHAIN, TNFSF4, TNFSF13, LTB, HGF, CCL28; Low: LYZ | Anti-tumor |
| CD206+ DC | High: MRC1, FCER1A, IFI30, IL18, FCGR2A, FCGR2B, FCGR2C, CD4, TNFSF12, TNFSF8 | Immunosuppressive | |
| CLEC7A+ DC | High: CLEC7A, ITGAE | Undefined | |
| CD1C+ DC | High: AREG, S100A8, S100A9, CD86, CD1C, CLEC9A, ANPEP, FBXO27 | Undefined | |
| CX3CR1+ DC | High: CX3CR1, CD8A, CD274, PDCD1LG2, TNFSF14, BTLA, CD70, TNFSF10, CCL5 | Immunosuppressive | |
| Mono/Mac | MS4A6Ahigh subset | High: MS4A6A, CD86, CSF1R, TGFB1, CCL22 | Immunosuppressive |
| CD163high subset | High: CD163, CXCR4, IL4R, TGFB1, CCL18 | Immunosuppressive | |
| MKI67+ subset | High: MKI67, VEGFA | Undefined | |
| MARCOhigh subset | High: IL1B, MARCO, CD4, CD68, CD86, CD14, CD163, IL4R, CSF1R, CX3CR1, TGFB1 | Immunosuppressive | |
| FCGR3A+ subset | High: CX3CR1, FCGR3A, CSF1R | Anti-tumor | |
| MRC1+ subset | High: MRC1, CSF1R, TGFB1 | Immunosuppressive | |
| CD14highITGAMhigh subset | High: CCR2, ITGAM, CD14 | Undefined | |
| CXCL8high subset | High: HIF1A, CXCL8 | Undefined | |
| IL10highTGFB1high subset | High: IL10, TGFB1 | Immunosuppressive | |
| T | CD4+ naïve T cell | High: CD4, CCR7, LEF1, TCF7, SELL | Dysfunctional |
| CD69highGZMA−CD4+ T cell | High: CD4, CD69; Low: GZMA | Anti-tumor | |
| CD69highGZMA+CD4+ T cell | High: CD4, CD69, GZMA | Anti-tumor | |
| CD4+ cytotoxic T | High: CD4, GZMA, GZMB, GZMH, GNLY, NKG7 | Anti-tumor | |
| TH17-like cell | High: CD4, RORC, KLRB1 | Dysfunctional | |
| Treg | High: CD4, IL2RA, FOXP3; Low: CD127 | Immunosuppressive | |
| Proliferating T cell | High: CD4, MKI67, PCNA, HAVCR2, LAG3, PRDM1, TIGIT, CTLA4, TOX | Dysfunctional | |
| IFN-CD4+ T cell | High: CD4, IFIT3, ISG15, ISG20, MX1, IFNAR1, LAG3, PRDM1, TIGIT | Dysfunctional | |
| GZMAlowGNLY+CD8+ T cell | High: CD8, GNLY; Low: GZMA | Anti-tumor | |
| TIGIT+CD8+ T cell | High: CD8, LAG3, TIGIT, GZMH, GZMA, GNLY, PRF1, GZMB | Dysfunctional | |
| Naïve CD8+ T | High: CD8, TCF7, SELL, LEF1, CCR7 | Undefined | |
| GZMAlowGNLYlowCD8+ T cell | High: CD8, CD69, RUNX3, IL7R; Low: KLRG1, ITGAE, B3GAT1, GZMA, GZMB, GZMH, GZMK, GNLY, PRF1, NKG7 | Anti-tumor | |
| GZMA+GNLYlowCD8+ T | High: CD8, GZMA, TIGIT, PDCD1, CTLA4 | Dysfunctional | |
| NK | NK cell | High: TYROBP, KLRF1, FCGR3A, NCAM1, CD160, GZMA, GNLY, PRF1, GZMB, TIGIT, HAVCR2; Low: CD3D, CD3E, CD8A, CD8B | Dysfunctional |