Figure 1.

Single-Cell RNA Sequencing reveals the presence of guide cells expressing CD44, Ng2, and Ncam2. (A) Flowchart showing the isolation of single cells from murine hind limbs with Smart-seq2 yielding cellular and phenotypic relationships. (B) UMAP plot and k-means clustering of 256 cells from murine skeletal muscle identifying five clusters, fibroblasts, Schwann cells, guide cells, activated satellite cells (MuSCs), and interstitial stromal cells (ISCs, as reported in [26]). Every point represents one cell. (C) Heatmap of k-means clusters of differentially expressed marker genes. (D) Violin plot with median visualizing marker genes (Cd44, Ng2, Ncam2, and Sox2) for the identified clusters.