Skip to main content
. 2021 Feb 16;22(4):1939. doi: 10.3390/ijms22041939

Figure 6.

Figure 6

Expression of neural markers in guide cells under different culture conditions. (A,D,G,J), Hoechst nuclei staining of CD34LOW (A,G) and CD34HIGH cells (D,J) cultured in neural medium (A,D) or D-MEM 2% Horse Serum (G,J); (B,E,H,K) anti-Tuj1 immunofluorescence of CD34LOW (B,H), and CD34HIGH cells (E,K) cultured in neural medium (B,E) or D-MEM 2% horse serum (H,K). Insets B’, E’, K’ represent sub-regions of panels B, E, K respectively; (C) merge of A and B panels; (F) merge of D and E panels; (I) merge of panels G and H; (L) merge of panels J and K. Scale bar (30 μm), in L, is the same for all the panels. (M) Connexin40 mRNA levels in the original population used as reference (C), after 5-azacytidine treatment (5-aza) and in the guide cells used in the experiments, grown both in proliferating medium and in low serum medium (CD34LOW diff and CD34HIGH diff cells). (N) Relative expression of Sox2 (white bar) and Sox17 (black bar) in cardiac mesoangioblasts (ctrl) or guide cells cultured for 24 hours (24h) in proliferating medium and 5 days in low serum medium (5 d); * p < 0.05, ** p < 0.01; **** p < 0.0001.