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Growth Curves of Parental Strains. BTV-2 and BTV-10 demonstrate similar growth kinetics in BHK 21 cells. BTV-2 or BTV-10 was used to initiate single-virus infections in duplicate on BHK 21 cells with a starting MOI of 0.2 TCID50/mL. Supernatant was collected at 1, 6, 12, 24, 48, and 72 h post infection and TCID50s were performed at each time point to determine infectious titer.
