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. 2021 Feb 16;13(2):310. doi: 10.3390/v13020310

Figure 1.

Figure 1

DENSpm induces polyamine depletion and limits Coxsackievirus B3 infection. (A) Schematic of the mammalian polyamine biosynthetic pathway. Pertinent inhibitors are highlighted in green, while important enzymes are highlighted in lilac. (B) Vero-E6 cells were treated with 10, 50, and 100 μM DENSpm for 16 h prior to collection for Western blot for SAT1, above, and thin layer chromatography for polyamines measuring the presence of putrescine (Put), spermidine (Spm), and spermine (Spm), below. (C) Cells were left untreated (NT) or treated with 100 μM DENSpm for 16h prior to infection at multiplicity of infection of 0.01 CVB3. Viral titers were determined by plaque assay at the times indicated. (D) Cells were treated with escalating doses of DENSpm for 16h prior to infection at MOI 0.01. Viral titers were determined at 48 hpi. ** p ≤ 0.01using Student’s t-test (n ≥ 3), comparing treated samples to untreated controls. Error bars represent ± 1 SEM.