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. 2021 Feb 16;13(2):310. doi: 10.3390/v13020310

Figure 5.

Figure 5

DENSpm reduces 2A protease activity. Split luciferase protease activity reporter systems were cloned and co-transfected with (A) wildtype 2A or (B) 2AS35G mutant into Vero cells left untreated or treated with increasing doses of DENSpm. Firefly luciferase activity was measured 24 h later and normalized to renilla luciferase transfection efficiency control and subsequently normalized to untreated cell transfection. (C) Vero cells were left untreated or treated with increasing doses of DENSpm for 16 h prior to infection with wildtype CVB3. Total cellular protein was collected 24 hpi and analyzed via Western blot for eIF4G and β-actin. ** p ≤ 0.01, *** p ≤ 0.001 using Student’s t-test (n = 2), comparing treated samples to untreated controls. Error bars represent ± 1 SEM.