Skip to main content
. 2021 Feb 16;10(2):404. doi: 10.3390/cells10020404

Figure 1.

Figure 1

Established cell system: (A) DJ-1 Immunoblot of SH-SY5Y cells overexpressing different DJ-1 variants or pMIG empty vector (B) Timeline for SH-SY5Y differentiation with retinoic acid (RA) and brain derived neurotrophic factor (BDNF) (C) Micrographs of undifferentiated and differentiated SH-SY5Y cells (scale bar: 100 µm) (D) Real-time SH-SY5Y cell proliferation over 7 days of RA + BDNF treatment depicted as cell index over time of three technical replicates was assessed by absorbance at 370 nm (E) BrdU incorporation of untreated and RA + BDNF treated SH-SY5Y cells measured as the absorbance at 370 nm wavelength. (F) Immunoblots of mature neuronal markers Growth-associated Protein 43 (GAP43) and β-3-Tubulin with GAPDH as loading control.