Figure 5.

Effect of DJ-1 on protein glycation: Effect on protein glycation by overexpression of (A) full length DJ-1 variants DJ-1^WT and DJ-1^C106A and (B) C-terminally truncated DJ-1 variants; left: Immunoblots of whole cell lysates of untreated and MGO treated cells expressing different DJ-1 variants with anti-MGO antibody and GAPDH as loading control: right: quantification of signal intensity of MGO-modified proteins relative to empty vector control (pMIG empty) with ImageJ of three independent experiments for full length variants and two independent experiments for C-terminally truncated variants (** p ≤ 0.01 by two tailed t-test on independent groups).