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. 2020 Dec 28;32(3):597–613. doi: 10.1681/ASN.2019101025

Figure 4.

Figure 4.

Autophagic activity was suppressed in the podocytes of Rpt3pdKO mice. (A) IF staining showed LC3 dots of Rpt3Control and Rpt3pdKO kidneys treated with vehicle (PBS) or chloroquine. Scale bar: 20 μm in A. (B) The area of LC3 dots colocalized with synaptopodin was smaller in the glomeruli of Rpt3pdKO mice than in Rpt3Control mice. The area of LC3 dots in Rpt3pdKO did not significantly change by chloroquine treatment (n=18, P=0.05 by Mann–Whitney U test). (C) The expression of p62 significantly increased in Rpt3pdKO mice in WB of glomerular lysate. (D) The densitometry quantification of p62 expression in WB shown in (C) (n=3, P=0.01 by Welch t test). (E) p62 was accumulated in the podocyte cell bodies of Rpt3pdKO mice and partially colocalized with ubiquitin at 4 weeks of age. Scale bars: 20 μm in low magnification of E; 2 μm in high magnification of E. (F) The area of p62 particles per glomerulus was significantly larger in Rpt3pdKO mice than in Rpt3Control mice at 4 weeks of age (n=3, P<0.001 by Mann–Whitney U test). *P=0.05; **P=0.01; ***P<0.001.