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Size Exclusion Chromatography (SEC)elution profiles of the retro proteins. Red: The rRop subjected to urea denaturation in the lysis buffer and subsequent refolding on a Ni-NTA column, prior to SEC purification. Blue: The rRop purified with a Ni-NTA column under non-denaturing conditions, prior to SEC purification. The protein was only detected in the second peak. Green: Elution profile of rRM6 purified under non-denaturing conditions with a Ni-NTA column, prior the SEC purification.
